Journal: Emerging Infectious Diseases

Article Title: Human Metapneumovirus, Peru

doi: 10.3201/eid1202.051133

Figure Lengend Snippet: Human metapneumovirus (HMPV)–positive samples by F- and N-gene primers

Article Snippet: A shell vial containing a near confluent monolayer of LLC-MK2 cells (Diagnostic Hybrids, Inc., Athens, OH, USA) was injected with 100 μL specimen and 900 μL HMPV growth media (1× minimum essential medium with L-glutamine and Earle salts, 0.1% bovine albumin, 1× HEPES, 0.001% porcine pancreatic trypsin, 0.4505 mol/L D-glucose, 10,000 U penicillin, 10 mg streptomycin, and 50 μg amphotericin), centrifuged for 1 h at 37°C and 2,800 rpm (1,500× g ), followed by a 37°C incubation with 5% CO 2 .

Techniques:

Journal: Molecules

Article Title: 2,5-Diketopiperazine Derivatives as Potential Anti-Influenza (H5N2) Agents: Synthesis, Biological Evaluation, and Molecular Docking Study

doi: 10.3390/molecules27134200

Figure Lengend Snippet: Summary of influenza virus (H5N2) propagation inhibition in embryonated chicken eggs of LS-C, LS-D, and 2,5-DKP derivatives detected by hemagglutination assay and the cytotoxicity activity (IC 50 ) against LLC-MK2.

Article Snippet: Cytotoxicity was evaluated against normal cell line Rhesus monkey kidney epithelial cells LLC-MK2 (Korean Cell Line Bank, KCLB) by using a MTT assay [ ].

Techniques: Virus, Inhibition, Hemagglutination Assay, Activity Assay

Journal: medRxiv

Article Title: Humoral cross-reactivity towards SARS-CoV-2 in young children with acute respiratory infection with low-pathogenicity coronaviruses

doi: 10.1101/2021.10.01.21264349

Figure Lengend Snippet: LLC MK2 cells were infected with HCoV-OC43 and at 48 h post-infection, cells were fixed and stained with 1:10 dilution of serum samples from indicated patients. A commercial antibody against the N protein is used as a positive control. Alexa-488 conjugated secondary antibody (either anti-human or anti-rabbit) was used to detect the primary antibody. Nuclei were stained by Hoechst stain. Scale 10 μm.

Article Snippet: LLC MK2 cells were from European Collection of Authenticated Cell Cultures Salisbury, UK (ECACC - 85062804).

Techniques: Infection, Staining, Positive Control

Journal: medRxiv

Article Title: Humoral cross-reactivity towards SARS-CoV-2 in young children with acute respiratory infection with low-pathogenicity coronaviruses

doi: 10.1101/2021.10.01.21264349

Figure Lengend Snippet: Huh-7 cells were infected with HCoV-229E and LLC MK2 cells were infected with HCoV-NL63 or HCoV-OC43 and at 48 h post-infection, cells were fixed and stained with 1:10 dilution of serum samples from a healthy or a positive subject. A commercial antibody against the N protein is used as an assay positive control. Alexa-488 conjugated secondary antibody (either anti-human or anti-rabbit) was used to detect the primary antibody. Nuclei were stained by Hoechst stain.

Article Snippet: LLC MK2 cells were from European Collection of Authenticated Cell Cultures Salisbury, UK (ECACC - 85062804).

Techniques: Infection, Staining, Positive Control